Download Antiviral Methods and Protocols by Derek Kinchington, Raymond F. Schinazi PDF

By Derek Kinchington, Raymond F. Schinazi

Royal London tuition of medication, united kingdom. moment writer, Raymond F. Schinazi, is at Emory Univ., Decatur, GA. bargains uncomplicated and scientific researchers types to judge compounds potent opposed to acute and protracted infections. contains complete assays, caliber checking out, and unpublished tools. DNLM: Hepatitis, Viral, Human - drug treatment.

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Morris, C. , and Melling, J. M. (1989) Animal cell culture collections and the supply of authenticated cultures and services for industry and research, in Biotechnology and Engineering Reviews, vol. 7, Intercept, Andover, UK. 2. Stacey G. , Stephenson, J. , and Doyle, A. (1997) Authentication of animal cell cultures by direct visualisation of DNA, Aldolase gene PCR and isoenzyme analysis. Biologicals 25, 75–83. 3. McGarrity, G. , and Vaidya, A. (1980) Mycoplasma infection of lymphocyte cultures: infection with M.

Wiley, New York, pp. 5. HBV Assays for Antiviral Activity 51 5 Hepatitis B Virus Cell Culture Assays for Antiviral Activity Karen Schmidt and Brent Korba 1. Introduction Hepatitis B Virus (HBV) is a causative agent of both acute and chronic hepatitis, a major etiologic factor of primary hepatocellular carcinoma, and a serious global health problem, with over 240 million estimated chronically infected individuals. Although there are several promising antiviral agents currently in clinical trials, alpha interferon remains the only licensed drug for the treatment of chronic HBV infection.

4 (molecular biology grade). Chloroform. HBV Assays for Antiviral Activity 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. 52. 53. 53 Isopropanol. Ammonium acetate. 25% bromophenol blue, 30% glycerol in TE. TAE (Tris-acetate-EDTA running buffer). Agarose (standard low–mr). Plastic pipets (1 mL to 50 mL, sterile, individually wrapped). 5 in. Glass Pasteur pipets. 96-Well tissue culture plates. 24-Well tissue culture plates. Tissue culture flasks (75, 150 cm2).

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