By Donald Parriott
This consultant for the practising chromatographer who wishes a prepared resource of data on HPLC detection explores and compares current detection platforms and detectors, outlines the typical difficulties linked to a given detector, and gives confirmed ways to heading off such difficulties.
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Extra info for A Practical Guide to HPLC Detection
3-7). b. Compensation for refractive index changes Another design consider ation is compensation for refractive index changes. Solutions with different refractive indices bend light to differing degrees, according to Snell's law (Fig. 3-8). When the refractive index of the mobile phase changes, either because of 46 Roger Blain hv INCIDENT LIGHT hv PARTIALLY SILVERED MIRROR FIG. 3-6 Beam splitter. gradient elution or due to a sample solvent different than the mobile phase eluting at the void volume of the column, a shift in the baseline occurs.
OVERVIEW OF ABSORBANCE DETECTORS A major class of HPLC detectors, called absorbance detectors, measures the loss in intensity of ultraviolet or visible light as it passes through the solution exiting an HPLC column. , single wavelength) light, a flow cell through which the light shines, and a means of measuring the change in the light intensity as it exits from the flow cell. A schematic representation is shown in Fig. 3-1. The source of monochromatic light can be either a lamp plus a simple filter or a lamp plus a monochromator, a device that allows one to select a single wavelength from a lamp source that emits light over a wide region of wave lengths.
In "Physical Methods of Organic Chemistry," (A. ), Vol. 1, part 2, Interscience, New York, p. 1193. Jenkins, F. , and White, H. E. (1976). " McGraw-Hill, New York, p. 479. "Lange's Handbook of Chemistry, eleventh edition," (1973). (J. A. ) McGraw-Hill, New York, p. 10-250. N. (1983). in "Liquid Chromatography Detectors," (T. M. ) Marcel Dekker, New York, Ch. 5. Scott, R. P. W. (1977). " Elsevier, New York, Ch. 2. Vandenheuvel, F. , and Sipos, J. C. (1961). Anal. Chem. 33, pp. 286-291. Waters, J.